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KMID : 1123920020160061284
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Korean Journal of Oriental Physiology and Pathology
2002 Volume.16 No. 6 p.1284 ~ p.1290
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Scrophularia Buergeriana inhibits the Production of NO through the Suppression of NF-kB adivity in LPS-stimulated Mouse Peritoneal Macrophages
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Ha Mi-Suk
Kim Young-Hee
Ko Woo-Shin
Kim Han-Do
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Abstract
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Scrophularia buergeriana Miquel (Scrophulariaceae) has been used as an anti-inflammatory drug in the folk medicine recipe and been proved its anti-inflammatory effect in the oriental medicine. Since nitric oxide (NO) and superoxide anion (O/sub 2//sup -/) are ones of the major inflammatory parameters, we studied the effect of aqueous extracts of Scrophularia buergeriana (SB) on NO and O/sub 2//sup -/ production in lipopolysaccharide (LPS)-stimulated mouse peritoneal macrophages. NO, O/sub 2//sup -/production and inducible NO synthase (iNOS) level were significantly reduced in LPS-activated macrophages by SB compared to those without. Electrophoretic mobility shift assay (EMSA) indicated that SB blocked the activation of NF-kB, which was considered to be a potential transcription factor for the iNOS expression. SB also blocked degradation of IkBα. Furthermore, IkB kinase alpha (IKKα), which phosphorylates serine residues of IkB directly, is inhibited by SB. These results suggest that SB could exert its anti-inflammatory actions by suppressing the activation of NF-kB through inhibition of IKK activity.
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KEYWORD
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Scrophularia buergeriana Mique, Superoxide, Nitric oxide (NO), Inducible nitric oxide synthase (iNOS), Nuclear Factor kappa B (NF-kB), Inhibitor kappa B(IkB), IkB kinase (IKK)
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